Basics of Image Processing for cryo-EM-Part II
This course provides an introduction to important concepts and practical workflows of cryo-electron microscopy (cryo-EM) image processing. Participants will learn the fundamentals of single-particle analysis, including motion correction, CTF estimation, particle picking, 2D classification, 3D reconstruction, and refinement. This second session consists of a hands-on tutorial in image processing performed in cisTEM. Participants should attend the first lecture prior to attending this course.
Axel Brilot (Facility Director, CBRS); Michelle Mikesh (EM and Sample Preparation Specialist)
Axel Brilot obtained his Ph.D. in Biophysics and Structural Biology developing and applying cryo-EM methods for single-particle reconstruction. He has 15 years’ experience in the cryo-EM field and has been the facility director of the Sauer Structural Biology Laboratory since 2021.
Michelle worked as a lab manager with more than 25 years of research experience before joining the CBRS in 2019. Since then, she has enjoyed assisting students and researchers with preparing and imaging samples from microbiology to materials engineering and looks forward to expanding her work with cryoEM.
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$50