Biomolecular Structure Determination by CryoEM
Transmission Electron Microscopy (TEM) and Cryogenic electron microscopy (cryo-EM)are versatile methods for biological structural characterization. Technological advancements made in the past decade have enabled cryo-EM to become an approachable high-resolution method capable of generating atomic structures.
The course will be divided into three sections: an initial 45-minute section on the room temperature (classical) EM services offered by the core; followed by two sections: an overview of the basic principles of single particle cryo-EM along the related services our core offers users, split into two approximately one hour sections with breaks between all sections. We will also discuss the practical applications of cryoEM and what a user will need to perform a successful experiment.
Axel Brilot (Facility Director, CBRS); Michelle Mikesh (EM and Sample Preparation Specialist)
Axel Brilot obtained his Ph.D. in Biophysics and Structural Biology developing and applying cryo-EM methods for single-particle reconstruction. He has 15 years’ experience in the cryo-EM field and has been the facility director of the Sauer Structural Biology Laboratory since 2021.
Michelle worked as a lab manager with more than 25 years of research experience before joining the CBRS in 2019. Since then, she has enjoyed assisting students and researchers with preparing and imaging samples from microbiology to materials engineering and looks forward to expanding her work with cryoEM.
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$10