This course provides an introduction to important concepts and practical workflows of cryo-electron microscopy (cryo-EM) image processing. Participants will learn the fundamentals of single-particle analysis, including motion correction, CTF estimation, particle picking, 2D classification, 3D reconstruction, and refinement. The course consists of two sessions, with the first session consisting of a lecture, and the second session consisting of a hands-on processing session. Students intending to attend the hands-on session should ensure they have also taken this first lecture.
In order to best prepare for this course, students should learn basic concepts in cryo-EM from a short online course on cryo-EM (e.g. Cryo-EM university), as the course will build on a basic knowledge of concepts presented there.
Secondary recommended video resources:
- Single Particle Cryo-EM Overview by Yifan Cheng – Explains the principles of single-particle cryo-EM and 3D reconstruction from 2D images. Watch on YouTube
- Introduction and Cryo-EM Fundamentals (Part 1 of 6) – Covers data collection, motion correction, CTF estimation, particle picking, and 2D/3D classification using CryoSPARC. Watch on YouTube
- S2C2 Cryo-EM Image Processing Workshop (Full Session) – A nearly 5-hour workshop covering motion correction, CTF estimation, particle picking, 2D classification, and ab initio reconstruction. Watch on YouTube
- Cryo-EM: Back to Basics (Chapter 1) – A concise introduction to sample preparation, imaging, and data processing fundamentals. Watch on YouTube
- Single-Particle Data Analysis Walkthrough – A step-by-step CryoSPARC workflow: motion correction, CTF estimation, particle picking, classification, and refinement. Watch on YouTube
If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.
For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.
Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).
$50