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FNT 1.104

Basics of Image Processing for cryo-EM-Part I

Member for

3 years 1 month
Full name
Gary Chiang

This course provides an introduction to important concepts and practical workflows of cryo-electron microscopy (cryo-EM) image processing. Participants will learn the fundamentals of single-particle analysis, including motion correction, CTF estimation, particle picking, 2D classification, 3D reconstruction, and refinement. The course consists of two sessions, with the first session consisting of a lecture, and the second session consisting of a hands-on processing session. Students intending to attend the hands-on session should ensure they have also taken this first lecture.

In order to best prepare for this course, students should learn basic concepts in cryo-EM from a short online course on cryo-EM (e.g. Cryo-EM university), as the course will build on a basic knowledge of concepts presented there.

Secondary recommended video resources:

  1. Single Particle Cryo-EM Overview by Yifan Cheng – Explains the principles of single-particle cryo-EM and 3D reconstruction from 2D images. Watch on YouTube
  2. Introduction and Cryo-EM Fundamentals (Part 1 of 6) – Covers data collection, motion correction, CTF estimation, particle picking, and 2D/3D classification using CryoSPARC. Watch on YouTube
  3. S2C2 Cryo-EM Image Processing Workshop (Full Session) – A nearly 5-hour workshop covering motion correction, CTF estimation, particle picking, 2D classification, and ab initio reconstruction. Watch on YouTube
  4. Cryo-EM: Back to Basics (Chapter 1) – A concise introduction to sample preparation, imaging, and data processing fundamentals. Watch on YouTube
  5. Single-Particle Data Analysis Walkthrough – A step-by-step CryoSPARC workflow: motion correction, CTF estimation, particle picking, classification, and refinement. Watch on YouTube
Course ID
CB26008
Status
Open
Modality
Hybrid, but in-person encouraged
Course Closes
Wed, Nov 05
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Mon, Nov 10
10:00 am - 1:00 pm

$50

Overview of the Biological Mass Spectrometry Facility

Member for

3 years 1 month
Full name
Gary Chiang

The class will be an overview of the Biological Mass Spectrometry Facility covering staff, equipment, services, and collaborative opportunities. New equipment for proteomics and metabolomics will be highlighted.

Course ID
CB26012
Status
Open
Modality
Hybrid (In-person or Zoom)
Course Closes
Wed, Oct 29
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Mon, Nov 03
10:00 am - 11:00 am

Free

Best Practices in Mouse Colony Management

Member for

3 years 1 month
Full name
Gary Chiang

The goal of this course is to provide an overview of best practices for maintaining genetically engineered mouse colonies. Topics will include mouse reproductive basics, genetic validation, colony sizing, record keeping, and genetic drift.

Preferred or Prerequisite Skills:
No prerequisites, although an understanding of basic genetics is presumed.

Course ID
CB26006
Status
Open
Modality
In-person
Course Closes
Mon, Oct 20
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Fri, Oct 24
9:30 am - 12:30 pm

$10

Data Visualization using R

Member for

3 years 1 month
Full name
Gary Chiang

This course introduces both principles and practice of scientific data visualization, especially as applied to large multivariate data sets. Will cover common methods of visually summarizing data and illustrating relationships between variables of various common types (continuous, categorical, etc.) as well as design concepts for increasing the clarity of quantitative graphical communication. Will introduce modern “grammar of graphics” ideas as foundation for thinking about, relating, and ultimately building new types of informative plots. Implementations of covered methods in R will be presented. Students should bring their own laptops to the course with R and the associated packages dplyr, ggplot2 and pheatmap installed.

Preferred or Prerequisite Skills:
Some prior knowledge of R is required to get the most out of this class. The “Introduction to R” class would be useful for those not already comfortable with R programming prior to this course.

Course ID
CB26011
Status
Open
Modality
Hybrid, but in-person encouraged
Course Closes
Wed, Nov 26
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Mon, Dec 01
9:30 am - 12:30 pm

$50

Introduction to R

Member for

3 years 1 month
Full name
Gary Chiang

This course will introduce the fundamentals of programming in R. Topics will include coding guidelines, data types, functions, reading/writing files, and data manipulations within dataframes. We will also step into the world of the Tidyverse and learn how to manipulate dataframes within this new paradigm. This course is designed for students with little to no programming experience (prior installation of R is not required). The goal of this course is to become comfortable working in an R environment.

Preferred or Prerequisite Skills:
Students are expected to bring their own laptop and are able to connect to the UT WiFi network. As an introductory course, no prior knowledge of R programming is required.

Course ID
CB26010
Status
Open
Modality
Hybrid, but in-person encouraged
Course Closes
Wed, Nov 12
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Mon, Nov 17
9:30 am - 12:30 pm

$50

Introduction to RNA-seq

Member for

3 years 1 month
Full name
Gary Chiang

This is a theory course that will introduce some basics (both in experimental design and bioinformatics) that need to be considered when doing an RNA-Seq experiment. We will discuss library prep options, quality assessment, and bioinformatics analysis pipelines. We will also talk about analysis of single-cell and 3′ targeted RNA-Seq data. This course is designed to give you an idea of the options that are available when designing an RNA-Seq study or analyzing an RNA-Seq data set.

Course ID
CB26007
Status
Open
Modality
Hybrid (In-person or Zoom)
Course Closes
Wed, Oct 22
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Mon, Oct 27
9:30 am - 12:30 pm

$50

Introduction to Next Generation Sequencing

Member for

3 years 1 month
Full name
Gary Chiang

This course provides a high-level introduction to concepts and best practices for Next Generation Sequencing (NGS) analysis. Participants will gain familiarity with NGS vocabulary and file formats as well as popular tools commonly used in early processing. We will touch on the main skills and resources you need to get started, and aim to help you better understand what it takes to bridge the bench-scientist-to-bioinformatician divide.

Preferred or Prerequisite Skills:
Basic familiarity with DNA and RNA.

Course ID
CB26005
Status
Open
Modality
Hybrid (In-person or Zoom)
Course Closes
Wed, Oct 15
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Mon, Oct 20
9:00 am - 1:00 pm

$50

Python for Machine Learning/AI

Member for

3 years 1 month
Full name
Gary Chiang

Building further on the concepts covered in the Introduction, Intermediate, and Data Science Python courses, we will introduce Python as a tool for training and testing machine learning (ML) models with a particular focus on deep learning approaches. Specific topics will include an introduction to the PyTorch software library and a brief survey of some of the basic model architectures which it implements. Some prior familiarity with the basic ideas of ML (underfitting vs. overfitting, use of training and test data sets, etc.) and/or linear algebra will be helpful for getting the most out of this course.

Course ID
CB26004
Status
Open
Modality
Hybrid (In-person or Zoom)
Course Closes
Mon, Oct 13
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Fri, Oct 17
9:30 am - 12:30 pm

$50

Python for Data Science

Member for

3 years 1 month
Full name
Gary Chiang

This course will build up on the concepts covered in the Introduction to Python and Intermediate Python courses. We will introduce the use of Pandas Data frames to read in, subset, analyze and visualize RNA-Seq gene expression data.

Course ID
CB26003
Status
Open
Modality
Hybrid, but in-person encouraged
Course Closes
Wed, Oct 08
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Mon, Oct 13
9:30 am - 12:30 pm

$50

Intermediate Python

Member for

3 years 1 month
Full name
Gary Chiang

This domain non-specific course is designed for Python programmers who have basic experience with the language. Learners are expected to be familiar with control flow and basic Python data structures (variable assignment, lists, dictionaries). This course will cover the knowledge to make code modular, readable and reproducible. A major focus will be object-oriented programming and Python’s implementation of the object-oriented paradigm.

Course ID
CB26002
Status
Open
Modality
Hybrid, but in-person encouraged
Course Closes
Mon, Oct 06
Procard Disclaimer

If you use the UT ProCard for payment of courses, please be aware that you can only charge ONCE per 24 hour period. Any attempts to charge more courses will fail, and you will not be registered.

For example, you may add one to many courses for one student into your shopping cart at any one time, and charge them to the ProCard, and you should receive a "registration successful!" page at the end. This is because you registered ONCE for ONE student. If you attempt to register and pay again, for example, for a different student, this will trigger the UT ProCard security system to stop payment, and your registration will not be successful. A page stating this fact will occur after you attempt to process payment. It looks a lot like the "registration was successful" page.

Ways to avoid this are: use the ProCard after 24 hours have passed, or the student may use their credit card and be reimbursed later through the usual UT accounting methods, or process the registration with an IDT, otherwise known as an Interdepartmental Transfer (talk to someone in your department that handles the accounts).

Course Semester
Fri, Oct 10
9:30 am - 12:30 pm

$50

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