Nikon High Content Analysis

The Nikon High Content Analysis microscope is an automated inverted microscope for high-speed imaging of standard slides, culture dishes, and multi-well plates. It supports fluorescence, DIC, phase, and brightfield imaging with focus control and incubation capabilities for live cell experiments. The HCA features a monochromatic camera for widefield fluorescence imaging and a color camera for brightfield and DIC imaging.

Specifications:

• Objectives:
  • Plan Apo λ 4x; NA = 0.2, WD = 20 mm
  • Plan Apo λ 10x; NA = 0.5, WD = 4 mm
  • Plan Apo λ 20x; NA = 0.8, WD = 1 mm
  • Plan Apo λ 40x; NA = 0.9, WD = 0.25 mm
  • S Plan Fluor ELWD 20x Ph1; NA = 0.5, WD = 8.2 mm
  • S Plan Fluor ELWD 40x Ph2; NA = 0.6, WD = 3.6 mm
• Filters for fluorescence emission:
  • Multiband quad filter (blue, green, red, and far red) and multiband triple filter (CFP, YFP, mCherry)
  • Individual emission filters: DAPI, CFP, GFP, YFP, TxRed, Cy5
• Monochromatic Photometrics Prime 95B sCMOS camera
• Color Nikon DS-Ri2 camera
• JOBS automated acquisition macros
• Piezo motorized stage
• Focus control for long-term imaging
• Stage-top incubation
• Inverted microscope stand
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates
• ELWD objectives can be used with standard plastic bottom dishes or plates 

Applications:

• Widefield fluorescence microscopy
• Live cell imaging
• Time-lapse imaging
• Automated imaging and analysis of multiwell plates
• Brightfield microscopy
• Differential Interference Contrast (DIC)
• Color imaging
• Motorized stage
• Tiling of large image areas
• Phase contrast
• BSL-2 samples