Nikon AXR-NSPARC

The Nikon AXR-NSPARC is an inverted laser scanning confocal microscope that provides high-resolution fluorescence imaging and volume reconstructions. This microscope is also equipped with a Nikon Spatial Array Confocal (NSPARC) detector that allows super-resolution imaging of standard fluorescence samples down to 100 nm lateral resolution and 300 nm axial resolution.

Specifications:

• Objectives:
  • Plan Apochromat λ D 2x; NA - 0.1; WD - 8.5 mm
  • Plan Apochromat λ D 4x; NA - 0.2; WD - 20 mm
  • Plan Apochromat λ D 10x; NA - 0.45; WD - 4.0 mm
  • Plan Apochromat λ D 20x; NA - 0.8; WD - 0.8 mm
  • Plan Apochromat λ S 40x (Silicone); NA - 1.25; WD - 0.3 mm
  • Plan Apochromat λ D 60x (Water); NA - 0.127; WD - 0.18 mm
  • Plan Apochromat λ D 60x (Oil); NA – 1.42; WD - 0.15 mm
• Lasers: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm
• Tunable spectral emission detection for confocal imaging
• Filters for NSPARC emission detection: blue, green, red, far red, and a quad bandpass filter
• Resolution with NSPARC: 100 nm lateral and 300 nm axial
• High-speed resonant scanner
• Piezo motorized stage
• Focus control for long-term imaging
• Stage-top incubation
• Inverted microscope stand
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates

Applications:

• Confocal microscopy
• Live cell imaging
• Super-resolution microscopy
• Z-stacking
• Spectral unmixing
• Time-lapse imaging
• Differential Interference Contrast (DIC)
• Transmitted light imaging
• Organoid imaging
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence resonant energy transfer (FRET)
• Tiling of large image areas
• Fluorescence correlative spectroscopy
• BSL-2 samples