Nikon AXR 8-Laser

The Nikon AXR is an inverted laser scanning confocal microscope that provides high-resolution fluorescence imaging and volume reconstructions. This AXR is equipped with eight excitation lasers and spectral emission for better separation of fluorescence. It is located in the Health Discovery Building.

Specifications:

• Objectives:
  • Plan Apo λ D 4x; NA - 0.2; WD - 20 mm
  • Plan Apo λ 10x; NA - 0.45; WD - 4 mm
  • Plan Apo λ 20x; NA - 0.75; WD - 1 mm
  • Plan Fluor DIC 40x (Oil); NA - 1.3; WD - 0.24 mm
  • Plan Apo λ 60x (Oil); NA - 1.4; WD - 0.14 mm
• Lasers: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm, 730 nm
• Tunable spectral emission detection for confocal imaging
• High-speed resonant scanner
• Piezo motorized stage
• Focus control for long-term imaging
• Inverted microscope stand
• Compatible with glass slides, 35 mm dishes, and multi-well plates

Applications:

• Confocal microscopy
• Near-infrared fluorescence
• Z-stacking
• Time-lapse imaging
• Differential Interference Contrast (DIC)
• Transmitted light imaging
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence resonant energy transfer (FRET)
• Tiling of large image areas
• BSL-2 samples