The Nikon A1R dual beam large format multiphoton microscope uses infrared excitation to image through thick samples, including intravital imaging of multiple animal species. It has a large stage area with an open architecture to accommodate large stereotactic equipment, treadmills and other devices. It uses an upright microscope configuration with water dipping objectives for imaging with the front lens immersed in the sample media.
Specifications:
- Objectives:
- CFI75 Apo 25X Water dipping; NA = 1.1, WD = 1.43-2.04 mm
- CFI75 LWD 16X Water dipping; NA=0.8, WD=3.0 mm
- CFI90 APO 20X C Glycerin; NA=1.00, WD=8.2 mm
- Visible lasers for confocal imaging: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm
- Infrared lasers: 680 – 1300 nm tunable and 1040 nm
- High-speed resonant scanner
- Tunable spectral emission detection for confocal imaging
- Filters for multiphoton imaging with IR lasers: 440/80, 550/88, 641/75, and 735/68
- Upright microscope stand
- Motorized stage
- Compatible with glass slides, 35 mm dishes, 60 mm dishes, 6- and 12-well plates
- Flat stage insert for intravital imaging or non-standard samples
Applications:
- Multiphoton microscopy
- Confocal microscopy
- Intravital microscopy
- Water dipping objective
- Z-stacking
- Spectral unmixing
- Laser ablation
- Fluorescence resonant energy transfer (FRET)
- Tiling of large image areas
- Cleared tissue imaging
- NIDAQ control of external devices
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