The Orbitrap Fusion Tribrid Mass Spectrometer coupled with Ultimate 3000 RSLCnano UPLC (left) for proteomics applications or Ultimate 3000 microflow LC (right) for intact protein molecular weight determination.
Applications
- Protein & Peptide Analysis: Molecular weight determination and MS/MS and MSn analysis using CID and HCD
- Protein Identification: Supports gel bands, gel sections, and complex mixtures
- Quantitation Methods: Spectral counting, stable isotope labeling, intensity-based quantitation, and data-independent acquisition
- PTM Analysis: Comprehensive post-translational modification characterization
- High-Throughput Proteomics: Large-scale applications supported by Evosep One for increased throughput
Features
Thermo Obitrap Fusion Tribrid Mass Spectrometer
- Mass Analyzers: Quadrupole, Linear ion trap and Orbitrap mass analyzers
- Detectors: Ion Trap, Orbitrap mass analyzer
- Fragmentation/Activation: CID and HCD
Ultimate 3000 RSLCnano UPLC
- Nanoflow ultra high pressure liquid chromatography
- Autosampler loading from vials or 96 well plates
Ultimate 3000 RSLC UPLC
- Microflow UPLC up to 200 ul/min
- Autosampler loading from vials
Proteomics Software
- Proteome Discoverer: Database search algorithms for protein identification, quantitation, and modification analysis
- Spectronaut: DIA-based identification and quantitation
- Scaffold: Data visualization and validation for protein analysis
- Byonic: Protein modification analysis
- PEAKS: de novo sequencing
Is Ongoing
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