The TIRF/STORM microscope has a single inverted microscope stand that functions as both a TIRF microscope and super-resolution STORM microscope. TIRF microscope acquires high-resolution fluorescence images of thin samples. STORM can be used for super-resolved detail of thin samples. Fast two-color imaging can be done with the dual camera configuration. High-resolution single color images can be acquired with the sCMOS camera.
Specifications:
- Objectives:
- Plan Apochromat λ 10x; NA - 0.45; WD - 4.0 mm
- Plan Apochromat λ 20x; NA - 0.75; WD - 1.0 mm
- Plan Apochromat λ D 40x; NA - 0.95; WD - 0.17- 0.25mm
- Plan Fluor 40x (Oil); NA - 1.3; WD - 0.20 mm
- Plan Apochromat λ 60x (Oil); NA - 1.4; WD - 0.13 mm
- CFI HP APO TIRF Auto Correction Collar 100x (Oil); NA - 1.49; WD - 0.12 mm
- CFI SR HP Apo TIRF 100x (Oil); NA - 1.49; WD - 0.12 mm
- Lasers: 405 nm, 488 nm, 561 nm, 640 nm
- Filter for fluorescence emission: Multiband quad filter (blue, green, red, and far red)
- Resolution with STORM: 20 nm lateral and 50 nm axial
- Andor iXon Life DU-897 monochromatic EMCCD cameras
- Orca Flash 4.0 V3 monochromatic sCMOS camera
- Nikon DS-FI3 color camera
- Dual camera configuration
- Splitters for two color imaging with dual cameras: Green-Red; Red-Far Red
- Piezo motorized stage
- Focus control for long-term imaging
- Stage-top incubation
- Inverted microscope stand
- Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates
Applications:
- Total Internal Reflectance Fluorescence (TIRF) microscopy
- Localization microscopy
- Super-resolution microscopy
- PALM, STORM, DNA-PAINT
- Live cell imaging
- Time-lapse imaging
- Differential Interference Contrast (DIC)
- Brightfield microscopy
- Color imaging
- BSL-2 samples
Is Ongoing
Off
Resource Image
