Library Preparation Services

The GSAF offers a broad range of library preparation services supporting diverse experimental designs and sequencing applications. Our standard workflows are optimized and validated, and we also welcome discussions around custom or emerging methods to support unique research needs.

If a service is not listed below, please contact the facility to discuss feasibility and experimental design.

RNA Sequencing

• Poly-A selection or ribosomal depletion for mRNA and total RNA profiling
• TagSeq (3’ mRNA) – cost-effective gene expression profiling
• Small RNA sequencing – miRNA and other small RNA species

DNA Sequencing

• Whole genome sequencing (WGS)
  • Enzymatic or mechanical fragmentation
• DNA Library preparation from user-prepared amplicons
• 16S / ITS metagenomics for microbial community profiling

Single-Cell Sequencing

10x Genomics platforms:

• 3’ or 5’ Gene Expression (GEX)
• On-chip multiplexing for efficient sample batching
• Multiome ATAC + GEX
• FLEX fixed RNA profiling for preserved samples

Parse Biosciences:

• Scalable workflows supporting 10,000 to 1 million cells
• Optional TCR or BCR profiling add-ons