Light Microscopy Instruments

The Nikon Ts2R is an inverted fluorescence microscope that is ideal for screening cells in standard plastic bottom dishes or plates. It has almost entirely manual control but is equipped with a motorized stage for automated sample positioning.

Specifications:

• Objectives:
  • Plan Fluor 4x PhL; NA = 0.1, WD = 16.5 mm
  • Plan Fluor 10x Ph1; NA = 0.3, WD = 16 mm
  • S Plan Fluor ELWD 20x Ph1; NA = 0.5, WD = 8.2 mm
  • S Plan Fluor ELWD 40x Ph2; NA = 0.6, WD = 3.6 mm
• Filters for fluorescence emission: Multiband quad filter (blue, green, red, and far red) and multiband triple filter (CFP, YFP, mCherry)
• Monochromatic Photometrics CoolSnap Dyno camera
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates
• ELWD objectives can be used with standard plastic bottom dishes or plates
• Motorized stage
• Inverted microscope stand

Applications:

• Widefield fluorescence microscopy
• Brightfield microscopy
• Phase contrast
• Imaging without coverslip glass bottom samples

The Nikon SMZ800N Stereoscope is useful for dissection or imaging of large samples or samples with non-typical mounting. It is capable of brightfield imaging with a color camera and uses low magnification objectives with additional zoom.

Specifications:

• Objectives:
• Nikon DS-Ri2 color camera
• Large field of view
• Manual stage
• 1 - 8x zoom

Applications:

• Dissection
• Color imaging
• Imaging of large and non-standard samples
• Color imaging
• Brightfield microscopy

This Nikon AXR is an upright laser scanning confocal microscope that provides high-resolution fluorescence imaging and volume reconstructions. Notably, this microscope has a water dipping objective, which allows imaging with the front lens immersed in the sample media.

Specifications:

• Objectives:
  • Plan Apochromat λ D 4x; NA - 0.2; WD - 20 mm
  • Plan Apochromat λ D 10x; NA - 0.45; WD - 4.0 mm
  • Plan Apochromat λ D 20x; NA - 0.8; WD - 0.8 mm
  • Plan Fluor 40x (Oil); NA - 1.3; WD - 0.2 mm
  • Plan Apochromat λ D 60x (Oil); NA – 1.42; WD - 0.15 mm
  • Plan Fluor λ D 10x (water dipping); NA - 0.3; WD - 3.5 mm
• Lasers: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm
• Tunable spectral emission detection
• High-speed resonant scanner
• Upright microscope stand
• Compatible with glass slides, 35 mm dishes, and 60 mm dishes

Applications:

• Confocal microscopy
• Water dipping objective
• Z-stacking
• Spectral unmixing
• Time-lapse imaging
• Differential Interference Contrast (DIC)
• Transmitted light imaging
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence resonant energy transfer (FRET)
• Tiling of large image areas
• Cleared tissue imaging

The Nikon Ni-E Upright Fluorescence Microscope features a monochromatic camera for widefield fluorescence imaging and a color camera for brightfield and DIC imaging.  Its upright configuration and motorized stage make it ideal for tiling of thin tissue sections.

Specifications:

• Objectives:
  • Plan Apo λ 4x; NA = 0.20, WD = 20 mm
  • Plan Apo λ 10x; NA = 0.45, WD = 4 mm
  • Plan Apo λ 20x; NA = 0.75, WD = 1 mm
  • Plan Apo λ 40x; NA = 0.95, WD = 0.25 mm
  • Plan Apo λ 60x Oil; NA = 1.40, WD = 0.13 mm
  • Plan Apo λ 100x Oil; NA = 1.45, WD = 0.13 mm
• Filters for fluorescence:
  • DAPI (ex 325-375, em 435-475)
  • FITC (ex 450-490, em 500-550)
  • TXRed (ex 530-560, em 590-650)
  • Cy5 (ex 590-650, em 663-738)
• Monochromatic Zyla 4.2 camera
• Color Nikon DS-Fi3 camera
• Upright microscope stand
• Compatible with glass slides

Applications:

• Widefield fluorescence microscopy
• Differential Interference Contrast (DIC)
• Brightfield microscopy
• Color imaging
• Motorized stage
• Tiling of large image areas

The Nikon SMZ25 motorized stereoscope provides fluorescence and brightfield imaging of macro and micro samples. It uses low magnification objectives with additional zoom for imaging larger or non-standard sample types.

Specifications:

• Objectives:
  • P2-SHR Plan Apo 0.5x, NA = 0.078, WD = 71 mm
  • P2-SHR Plan Apo 1.6X, NA = 0.25, WD = 30 mm
• Filters for fluorescence:
  • DAPI (ex 325-375, em 435-485)
  • CFP (ex 426-446, em 460-500)
  • GFP (ex 450-490, em 500-550)
  • GFP longpass (ex 450-490, em 500-∞)
  • YFP (ex 490-510, em 520-550)
  • DsRed (ex 530-560, em 590-650)
• Nikon DS-Ri2 color camera
• Large field of view
• Manual stage
• 0.63 - 15.75x zoom

Applications:

• Widefield fluorescence microscopy
• Imaging of large and non-standard samples
• Color imaging
• Brightfield microscopy
• Time-lapse imaging
• BSL-2 samples

The Nikon AXR-NSPARC is an inverted laser scanning confocal microscope that provides high-resolution fluorescence imaging and volume reconstructions. This microscope is also equipped with a Nikon Spatial Array Confocal (NSPARC) detector that allows super-resolution imaging of standard fluorescence samples down to 100 nm lateral resolution and 300 nm axial resolution.

Specifications:

• Objectives:
  • Plan Apochromat λ D 2x; NA - 0.1; WD - 8.5 mm
  • Plan Apochromat λ D 4x; NA - 0.2; WD - 20 mm
  • Plan Apochromat λ D 10x; NA - 0.45; WD - 4.0 mm
  • Plan Apochromat λ D 20x; NA - 0.8; WD - 0.8 mm
  • Plan Apochromat λ S 40x (Silicone); NA - 1.25; WD - 0.3 mm
  • Plan Apochromat λ D 60x (Water); NA - 0.127; WD - 0.18 mm
  • Plan Apochromat λ D 60x (Oil); NA – 1.42; WD - 0.15 mm
• Lasers: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm
• Tunable spectral emission detection for confocal imaging
• Filters for NSPARC emission detection: blue, green, red, far red, and a quad bandpass filter
• Resolution with NSPARC: 100 nm lateral and 300 nm axial
• High-speed resonant scanner
• Piezo motorized stage
• Focus control for long-term imaging
• Stage-top incubation
• Inverted microscope stand
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates

Applications:

• Confocal microscopy
• Live cell imaging
• Super-resolution microscopy
• Z-stacking
• Spectral unmixing
• Time-lapse imaging
• Differential Interference Contrast (DIC)
• Transmitted light imaging
• Organoid imaging
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence resonant energy transfer (FRET)
• Tiling of large image areas
• Fluorescence correlative spectroscopy
• BSL-2 samples

The Zeiss Axiovert 200M is a motorized, inverted microscope for imaging fixed samples like histological slides or live cells. It supports widefield fluorescence imaging, brightfield, and DIC.

Specifications:

• Objectives:
  • Fluar 5x; NA = 0.25, WD = 12.5
  • EC Plan-Neofluor 10x; NA = 0.30, WD = 5.2 mm
  • Plan-Apochromat 20x; NA = 0.80, WD = 0.55 mm
  • Plan-Apochromat 40x Oil; NA = 1.4, WD = 0.21 mm
  • Plan-Apochromat 63x Oil; NA = 1.4, WD = 0.19 mm
• Filters for fluorescence:
  • DAPI (ex 379-401, em 441-491)
  • CFP (ex 423-449, em 460-500)
  • FITC (ex 455-495, em 505-555)
  • GFP (ex 450-490, em 500-550)
  • YFP (ex 487-513, em 520-550)
  • TRITC (ex 540-580, em 592-668)
  • CY5 (ex 625-655, em 665-715)
  • CFP/YFP FRET (ex 426-446, em 520-550)
• Monochromatic AxioCam HRm camera
• Inverted microscope stand
• Motorized stage
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates

Applications: 

• Widefield fluorescence microscopy
• Brightfield microscopy
• Differential Interference Contrast (DIC)
• Fluorescence resonant energy transfer (FRET)

The Nikon A1R dual beam large format multiphoton microscope uses infrared excitation to image through thick samples, including intravital imaging of multiple animal species. It has a large stage area with an open architecture to accommodate large stereotactic equipment, treadmills and other devices.  It uses an upright microscope configuration with water dipping objectives for imaging with the front lens immersed in the sample media.

Specifications:

• Objectives:
  • CFI75 Apo 25X Water dipping; NA = 1.1, WD = 1.43-2.04 mm
  • CFI75 LWD 16X Water dipping; NA=0.8, WD=3.0 mm
  • CFI90 APO 20X C Glycerin; NA=1.00, WD=8.2 mm
• Visible lasers for confocal imaging: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm
• Infrared lasers: 680 – 1300 nm tunable and 1040 nm
• High-speed resonant scanner
• Tunable spectral emission detection for confocal imaging
• Filters for multiphoton imaging with IR lasers: 440/80, 550/88, 641/75, and 735/68
• Upright microscope stand
• Motorized stage
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, 6- and 12-well plates
• Flat stage insert for intravital imaging or non-standard samples 

Applications: 

• Multiphoton microscopy
• Confocal microscopy
• Intravital microscopy
• Water dipping objective
• Z-stacking
• Spectral unmixing
• Laser ablation
• Fluorescence resonant energy transfer (FRET)
• Tiling of large image areas
• Cleared tissue imaging
• NIDAQ control of external devices

The TIRF/STORM microscope has a single inverted microscope stand that functions as both a TIRF microscope and super-resolution STORM microscope. TIRF microscope acquires high-resolution fluorescence images of thin samples. STORM can be used for super-resolved detail of thin samples. Fast two-color imaging can be done with the dual camera configuration.  High-resolution single color images can be acquired with the sCMOS camera.

Specifications:

• Objectives:
  • Plan Apochromat λ 10x; NA - 0.45; WD - 4.0 mm
  • Plan Apochromat λ 20x; NA - 0.75; WD - 1.0 mm
  • Plan Fluor 40x (Oil); NA - 1.3; WD - 0.20 mm
  • Plan Apochromat λ 60x (Oil); NA - 1.4; WD - 0.13 mm
  • CFI HP APO TIRF Auto Correction Collar 100x (Oil); NA - 1.49; WD - 0.12 mm
  • CFI SR HP Apo TIRF 100x (Oil); NA - 1.49; WD - 0.12 mm
• Lasers: 405 nm, 488 nm, 561 nm, 640 nm
• Filter for fluorescence emission: Multiband quad filter (blue, green, red, and far red)
• Resolution with STORM: 20 nm lateral and 50 nm axial
• Andor iXon Life DU-897 monochromatic EMCCD cameras
• Orca Flash 4.0 V3 monochromatic sCMOS camera
• Nikon DS-FI3 color camera
• Dual camera configuration
• Splitters for two color imaging with dual cameras: Green-Red; Red-Far Red
• Piezo motorized stage
• Focus control for long-term imaging
• Stage-top incubation
• Inverted microscope stand
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates 

Applications:

• Total Internal Reflectance Fluorescence (TIRF) microscopy
• Localization microscopy
• Super-resolution microscopy
• PALM, STORM, DNA-PAINT
• Live cell imaging
• Time-lapse imaging
• Differential Interference Contrast (DIC)
• Brightfield microscopy
• Color imaging
• BSL-2 samples

The Nikon Eclipse Ni compound light microscope is used for general transmitted light images. Several objectives are fitted with optics for DIC and phase. It uses a color camera and low to high magnification air objectives.

Specifications:

• Objectives:
  • Plan 2x; NA = 0.06
  • Plan Fluor 4x; NA = 0.13
  • Plan Fluor 10x Ph1, DIC N1; NA = 0.3,
  • Plan Fluor 20x; NA = 0.50
  • Plan Fluor 40x NA, Ph2; NA = 0.75
  • Plan Fluor 60x; DIC N2 NA = 0.85 NA   
• Nikon DS-Ri2 color camera
• Manual stage
• Upright microscope stand

Applications:

• Phase contrast
• Differential Interference Contrast (DIC)
• Brightfield microscopy
• Color imaging

The Nikon W1 spinning disk confocal microscope uses multiple pinholes for ultrafast confocal imaging. With fast acquisition and limited laser light exposure, a spinning disk is ideal for live cell imaging.

Specifications:

• Objectives:
  • Plan Fluor DLL Ph1 10x; NA - 0.3; WD - 16.0 mm
  • Super Plan Fluor Ph1 ELWD 20x; NA - 0.45; WD - 6.9-8.2 mm
  • Plan Apochromat λ 20x; NA - 0.75; WD - 1.0 mm
  • Super Plan Fluor ELWD 40x; NA - 0.6; WD - 2.8-3.6 mm
  • Plan Apochromat VC 60x (Water); NA - 1.20; WD - 0.28-0.31 mm
  • Plan Apochromat λ 100x (Oil); NA - 1.45; WD - 0.13 mm
• Lasers: 405 nm, 488 nm, 561 nm, 640 nm
• Yokogawa W1 spinning disk confocal head
• Filters for fluorescence emission: DAPI, GFP, Texas Red, Cy5
• Pinholes: 25 µm and 50 µm
• Andor iXon DU-888 monochromatic EMCCD cameras
• Dual camera configuration
• Piezo motorized stage
• Focus control for long-term imaging
• Stage-top incubation
• Inverted microscope stand
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates
• ELWD objectives can be used with standard plastic bottom dishes or plates 

Applications:

• Live cell imaging
• Z-stacking
• Time-lapse imaging
• Differential Interference Contrast (DIC)
• Phase contrast
• Brightfield microscopy
• Imaging without coverslip glass bottom samples
• BSL-2 samples

The Nikon High Content Analysis microscope is an automated inverted microscope for high-speed imaging of standard slides, culture dishes, and multi-well plates. It supports fluorescence, DIC, phase, and brightfield imaging with focus control and incubation capabilities for live cell experiments. The HCA features a monochromatic camera for widefield fluorescence imaging and a color camera for brightfield and DIC imaging.

Specifications:

• Objectives:
  • Plan Apo λ 4x; NA = 0.2, WD = 20 mm
  • Plan Apo λ 10x; NA = 0.5, WD = 4 mm
  • Plan Apo λ 20x; NA = 0.8, WD = 1 mm
  • Plan Apo λ 40x; NA = 0.9, WD = 0.25 mm
  • S Plan Fluor ELWD 20x Ph1; NA = 0.5, WD = 8.2 mm
  • S Plan Fluor ELWD 40x Ph2; NA = 0.6, WD = 3.6 mm
• Filters for fluorescence emission:
  • Multiband quad filter (blue, green, red, and far red) and multiband triple filter (CFP, YFP, mCherry)
  • Individual emission filters: DAPI, CFP, GFP, YFP, TxRed, Cy5
• Monochromatic Photometrics Prime 95B sCMOS camera
• Color Nikon DS-Ri2 camera
• JOBS automated acquisition macros
• Piezo motorized stage
• Focus control for long-term imaging
• Stage-top incubation
• Inverted microscope stand
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates
• ELWD objectives can be used with standard plastic bottom dishes or plates 

Applications:

• Widefield fluorescence microscopy
• Live cell imaging
• Time-lapse imaging
• Automated imaging and analysis of multiwell plates
• Brightfield microscopy
• Differential Interference Contrast (DIC)
• Color imaging
• Motorized stage
• Tiling of large image areas
• Phase contrast
• BSL-2 samples

The Nikon A1R is an inverted laser scanning confocal microscope that provides high-resolution fluorescence imaging and volume reconstructions. This confocal is equipped with filters for fluorescence emission collection, making it ideal for standard blue, green, red, and far-red fluorophores.

Specifications:

• Objectives:
  • Plan Apo λ 10x; NA - 0.45; WD - 4 mm
  • Plan Apo λ 20x; NA - 0.75; WD - 1 mm
  • Plan Fluor DIC 40x (Oil); NA - 1.3; WD - 0.24 mm
  • Plan Apo λ 60x (Oil); NA - 1.4; WD - 0.14 mm
• Lasers: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm
• Filters for fluorescence emission detection: DAPI, CFP, GFP, YFP, TRITC, Cy5
• High-speed resonant scanner
• Piezo motorized stage
• Focus control for long-term imaging
• Stage-top incubation
• Inverted microscope stand
• Compatible with glass slides, 35 mm dishes, 60 mm dishes, and multi-well plates

Applications:

• Confocal microscopy
• Live cell imaging
• Z-stacking
• Time-lapse imaging
• Differential Interference Contrast (DIC)
• Transmitted light imaging
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence resonant energy transfer (FRET)
• Tiling of large image areas
• BSL-2 samples