Flow Cytometry Instruments

Flow cytometry is a technology that analyzes multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity.

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Cell Analyzers

BD Accuri C6 Plus

Flow Cytometry, Cell Analyzer

BD Accuri C6 Plus

Flow Cytometry, Cell Analyzer

The Accuri C6 Plus is a traditional flow cytometer with 2 lasers and 6 parameters for detection, including forward and side scatter and 4 fluorescence parameters. It’s easy to use for smaller fluorescence panels. Sample acquisition and analysis is done using FACSDiva. This analyzer is located at DPRI.

Specifications:

Lasers: 488 nm and 640 nm
4 Fluorescence channels
Photo-Multiplier Tube detectors
24 tube sample rack
Compatible with 5 mL polystyrene tubes and 96-well plates

Applications:

DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Cytek Aurora

Flow Cytometry, Cell Analyzer

Cytek Aurora

Flow Cytometry, Cell Analyzer

The Cytek Aurora is a spectral flow cytometer equipped with 5 lasers and 64 channels of fluorescence detection. Sample acquisition and analysis is done using SpectroFlo software.

Specifications:

Lasers: 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm
64 Fluorescence Channels
Spectral separation of highly overlapping fluorophores
Avalanche photodiode detectors
Compatible with 5 mL polystyrene tubes and 96-well plates

Applications:

Large panels, up to 40 colors of fluorescence
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Cytek Northern Lights

Flow Cytometry, Cell Analyzer

Cytek Northern Lights

Flow Cytometry, Cell Analyzer

The Cytek Northern Lights is a spectral flow cytometer equipped with 3 lasers and 38 channels of fluorescence detection. It is also equipped with a unique Small Particle Detector that allows detection of particles down to 70 nm in size. Sample acquisition and analysis is done using SpectroFlo software.

Specifications:

Lasers: 405 nm, 488 nm and 640 nm
38 Fluorescence Channels
Spectral separation of highly overlapping fluorophores
Enhanced Small Particle Detector: 70 nm minimum resolution
Avalanche photodiode detectors
40 tube sample rack
Compatible with 5 mL polystyrene tubes and 96-well plates

Applications:

Large panels, up to 24 colors of fluorescence
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression
Small Particle Analysis
Extracellular Vesicles
Virus-like Particles

BD LSRFortessa SORP

Flow Cytometry, Cell Analyzer

BD LSRFortessa SORP

Flow Cytometry, Cell Analyzer

The Fortessa is a traditional flow cytometer with 4 lasers and 15 parameters for detection, including forward and side scatter and 13 fluorescence parameters. It’s capable of automated specimen handling of 96- and 384-well plates. Sample acquisition and analysis is done using FACSDiva.

Specifications:

Lasers: 405 nm, 488 nm, 561 nm and 640 nm
13 Fluorescence channels
Photo-Multiplier Tube detectors
FACSFlow Supply System
Compatible with 5 mL polystyrene tubes and 96- & 384- well plates

Applications:

DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Cell Sorters

BD FACSAria Fusion SORP

Flow Cytometry, Cell Sorter

BD FACSAria Fusion SORP

Flow Cytometry, Cell Sorter

The BD Fusion is a cell sorter with 5 lasers and 20 parameters for detection, including forward and side scatter and 18 fluorescence parameters. The Fusion sits inside a biosafety cabinet to allow for sorting of biohazardous samples. Sample acquisition and analysis is done using FACSDiva.

Specifications:

Lasers: 355 nm, 405 nm, 488 nm, 561 nm and 640 nm
18 Fluorescence channels
Photo-Multiplier Tube detectors
Nozzle sizes: 70, 85 and 100 µm
4-way sorting into 5 mL tubes
2-way sorting into 15 mL tubes
Plate sorting
Compatible with 5- or 15-mL polystyrene tubes and 6-, 12-, 24-, 48-, 96- and 384-well plates

Applications:

Cell sorting
Single cell sorting
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression
BSL-2 samples

Sony MA900

Flow Cytometry, Cell Sorter

Sony MA900

Flow Cytometry, Cell Sorter

The Sony MA900 is a highly automated cell sorter with 4 lasers and 14 parameters for detection, including forward and side scatter and 12 fluorescence parameters. It uses sorting chips for increased reliability and simplified setup.

Specifications:

Lasers: 405 nm, 488 nm, 561 nm and 640 nm
12 Fluorescence channels
Photo-Multiplier Tube detectors
4-way sorting into 5 mL tubes
2-way sorting into 15 mL tubes
Plate sorting
Compatible with 5- or 15-mL polystyrene tubes and 6-, 12-, 24-, 48-, 96- and 384-well plates
Sorting chip size – 100 µm

Applications:

Cell sorting
Single cell sorting
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Sony SH800

Flow Cytometry, Cell Sorter

Sony SH800

Flow Cytometry, Cell Sorter

The Sony SH800 is a highly automated cell sorter with 4 lasers and 8 parameters for detection, including forward and side scatter and 6 fluorescence parameters. It uses sorting chips for increased reliability and simplified setup.

Specifications:

Lasers: 405 nm, 488 nm, 561 nm and 640 nm
6 Fluorescence channels
Photo-Multiplier Tube detectors
2-way tube, multiwell plates, PCR tubes, slides
Plate sorting
Compatible with 0.5-mL, 1.5-mL, 5-mL, and 15-mL tubes and 96-well plates
Sorting chip size – 100 µm

Applications:

Cell sorting
Single cell sorting
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Flow Cytometry