Flow Cytometry Instruments

Flow cytometry is a technology that analyzes multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity.

Explore Light Microscopy Instruments

Cell Analyzers

BD Accuri C6 Plus

Flow Cytometry, Cell Analyzer

BD Accuri C6 Plus

Flow Cytometry, Cell Analyzer

The Accuri C6 Plus is a traditional flow cytometer with 2 lasers and 6 parameters for detection, including forward and side scatter and 4 fluorescence parameters. It’s easy to use for smaller fluorescence panels. Sample acquisition and analysis is done using FACSDiva. This analyzer is located at DPRI.

Specifications:

Lasers: 488 nm and 640 nm
4 Fluorescence channels
Photo-Multiplier Tube detectors
24 tube sample rack
Compatible with 5 mL polystyrene tubes and 96-well plates

Applications:

DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

BD LSR Fortessa SORP

Flow Cytometry, Cell Analyzer

BD LSR Fortessa SORP

Flow Cytometry, Cell Analyzer

The Fortessa is a traditional flow cytometer with 4 lasers and 15 parameters for detection, including forward and side scatter and 13 fluorescence parameters. It’s capable of automated specimen handling of 96- and 384-well plates. Sample acquisition and analysis is done using FACSDiva.

Specifications:

Lasers: 405 nm, 488 nm, 561 nm and 640 nm
13 Fluorescence channels
Photo-Multiplier Tube detectors
FACSFlow Supply System
Compatible with 5 mL polystyrene tubes and 96- & 384- well plates

Applications:

DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Cytek Aurora

Flow Cytometry, Cell Analyzer

Cytek Aurora

Flow Cytometry, Cell Analyzer

The Cytek Aurora is a spectral flow cytometer equipped with 5 lasers and 64 channels of fluorescence detection. Sample acquisition and analysis is done using SpectroFlo software.

Specifications:

Lasers: 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm
64 Fluorescence Channels
Spectral separation of highly overlapping fluorophores
Avalanche photodiode detectors
Compatible with 5 mL polystyrene tubes and 96-well plates

Applications:

Large panels, up to 40 colors of fluorescence
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Cytek Northern Lights

Flow Cytometry, Cell Analyzer

Cytek Northern Lights

Flow Cytometry, Cell Analyzer

The Cytek Northern Lights is a spectral flow cytometer equipped with 3 lasers and 38 channels of fluorescence detection. It is also equipped with a unique Small Particle Detector that allows detection of particles down to 70 nm in size. Sample acquisition and analysis is done using SpectroFlo software.

Specifications:

Lasers: 405 nm, 488 nm and 640 nm
38 Fluorescence Channels
Spectral separation of highly overlapping fluorophores
Enhanced Small Particle Detector: 70 nm minimum resolution
Avalanche photodiode detectors
40 tube sample rack
Compatible with 5 mL polystyrene tubes and 96-well plates

Applications:

Large panels, up to 24 colors of fluorescence
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression
Small Particle Analysis
Extracellular Vesicles
Virus-like Particles

Cell Sorters

BD FACSAria Fusion SORP

Flow Cytometry, Cell Sorter

BD FACSAria Fusion SORP

Flow Cytometry, Cell Sorter

The BD Fusion is a cell sorter with 5 lasers and 20 parameters for detection, including forward and side scatter and 18 fluorescence parameters. The Fusion sits inside a biosafety cabinet to allow for sorting of biohazardous samples. Sample acquisition and analysis is done using FACSDiva.

Specifications:

Lasers: 355 nm, 405 nm, 488 nm, 561 nm and 640 nm
18 Fluorescence channels
Photo-Multiplier Tube detectors
Nozzle sizes: 70, 85 and 100 µm
4-way sorting into 5 mL tubes
2-way sorting into 15 mL tubes
Plate sorting
Compatible with 5- or 15-mL polystyrene tubes and 6-, 12-, 24-, 48-, 96- and 384-well plates

Applications:

Cell sorting
Single cell sorting
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression
BSL-2 samples

Sony MA900

Flow Cytometry, Cell Sorter

Sony MA900

Flow Cytometry, Cell Sorter

The Sony MA900 is a highly automated cell sorter with 4 lasers and 14 parameters for detection, including forward and side scatter and 12 fluorescence parameters. It uses sorting chips for increased reliability and simplified setup.

Specifications:

Lasers: 405 nm, 488 nm, 561 nm and 640 nm
12 Fluorescence channels
Photo-Multiplier Tube detectors
4-way sorting into 5 mL tubes
2-way sorting into 15 mL tubes
Plate sorting
Compatible with 5- or 15-mL polystyrene tubes and 6-, 12-, 24-, 48-, 96- and 384-well plates
Sorting chip size – 100 µm

Applications:

Cell sorting
Single cell sorting
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Sony SH800

Flow Cytometry, Cell Sorter

Sony SH800

Flow Cytometry, Cell Sorter

The Sony SH800 is a highly automated cell sorter with 4 lasers and 8 parameters for detection, including forward and side scatter and 6 fluorescence parameters. It uses sorting chips for increased reliability and simplified setup.

Specifications:

Lasers: 405 nm, 488 nm, 561 nm and 640 nm
6 Fluorescence channels
Photo-Multiplier Tube detectors
2-way tube, multiwell plates, PCR tubes, slides
Plate sorting
Compatible with 0.5-mL, 1.5-mL, 5-mL, and 15-mL tubes and 96-well plates
Sorting chip size – 100 µm

Applications:

Cell sorting
Single cell sorting
Immunophenotyping
DNA Cell Cycle / Ploidy
Apoptosis
Cell Proliferation
Fluorescent Protein Expression

Flow Cytometry

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