Interfacing Narrow Capillaries to MS via Electrospray Ionization Utilizing a Novel Porous Tip

Life Sciences : Research Tools

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  • Mehdi Moini, Ph.D. , University of Texas at Austin

Background/unmet need

Researchers rely on liquid chromatography (LC) and/or capillary electrophoresis (CE) methodologies to separate complex biological samples. The analysis and identification of these constituents, present at low quantity and resulting from limited sample volume, is usually through various mass spectrometry (MS) strategies. Because most MS methods are concentration sensitive, narrower interfaces between LC/CE devices and the MS are required for accurate analysis. However, at flow rates less than 30 µm-i.d., present interfaces generally fail.

Invention Description

Researchers at the University of Texas at Austin have developed a novel porous tip design for interfacing capillaries that simplifies the exchange of materials between separation devices and the MS units. This design has several marked advantages: 1) optimal performance is observed under nanoflow conditions; 2) no dead volume associated with design; 3) due to design, redox reactions with water at high voltage does not affect separation or MS performance; 4) design allows automated production of reproducible interface capillaries; 5) the same interfacing capillary is compatible with CE or LC source materials; 6) interface can be used with existing electrospray ionization source from a variety of manufacturers; 7) interface has been successfully used in the analysis of amino acids, peptides and intact proteins.

IP Status

  • 4 foreign patents application filed
  • 5 foreign patents issued
  • 1 U.S. patent issued: 8,198,586