Short, Synthetic Terminators for Controlling Fungal Gene Expression

Life Sciences : Research Tools

Available for non-exclusive licensing

Inventors

  • Hal Alper, Ph.D. , Chemical Engineering
  • Kathleen Curran , University of Texas at Austin

Background/unmet need

Terminators are key components of synthetic expression cassettes that are used in the fields of metabolic engineering, gene expression, protein production, and many others. However, in fungal systems very little effort has been made to develop synthetic terminators that help create more efficient expression cassettes; instead, current expression cassettes rely on native terminators. This untapped resource could potentially increase control over gene expression, protein production, and many other factors.

Invention Description

Researchers at The University of Texas at Austin have developed a line of short, synthetic terminators for fungal systems that are distinctly different from and more potent than native terminators. These terminators increase the net RNA from a promoter, thus allowing control over the gene expression of the fungal systems. This tool has been shown to work in two different fungal organisms and is likely applicable to all fungal systems.

Benefits/Advantages

  • No homology to native genome sequences
  • Short sequences (less than 100bp) 
  • Highly active (2 to 4 times stronger than native terminators)

Features

    Novel and active synthetic terminators for fungal systems 

Market potential/applications

Synthetic biology community; biofuels Industry; chemical Industry

Development Stage

Lab/bench prototype

IP Status