The Macromolecular Crystallography Facility allows users to solve the three-dimensional structures of macromolecules, using X-ray crystallography. The capacities of this center have recently been expanded into a modern core facility. The facility (also called the “X-ray Core”) now includes two state of the art detection systems, featuring the Rigaku MicroMax-007HF X-ray generator, two Rigaku R-AXIS IV++ image plate detectors, and Oxford cryo-cooling systems. The Rigaku MicroMax 007HF generator is equipped with two detectors, one mounted with the VariMax HighRes optics and the other with the VariMax HighFlux optics. The HighRes optics facilitate data collection on crystal unit cells up to 300 Å in size, and the HighFlux optics provide some of the strongest radiation outside of synchrotron sources. Cryo-cooling is available for both detectors; greatly facilitating the collection of high resolution data.
The facility also contains an Art Robbins Instruments Phoenix liquid-handling robot. It uses extremely small volumes, as small as 50 nanoliters, and is ideal for high throughput crystallization experiments. The core facility is also equipped with the Art Robbins CrysCam for automated visualization of crystallization experiments.
Four dual core HP workstations, installed with current popular crystallographic software, are available for diffraction data processing and structure determination. Storage of diffraction data is supported with a 2 terabyte RAID5 file system mounted on a Dell PowerEdge T300 server.
The Macromolecular Crystallography Facility is staffed to carry out structural analysis on a service basis, or to train and assist interested users in both crystallization and the collection, processing, and interpretation of X-ray diffraction data.