Sectioning & Microdissection

Thermo Scientific CryoStar NX50 Cryostat

The Thermo Scientific CryoStar NX50 cryostat is used for thick and thin sectioning of cryo-preserved and embedded specimens. The instrument will cut sections of 0.5 microns up to 500 microns and at temperatures as low as -45 Celsius.

Instrument Access: Training for individual use is available. To schedule training, please fill out the Access Request Form and email it to Michelle Mikesh.

Fees: Trained users: $7/hour

Leica UltraCut Ultramicrotome

The Leica UCT is used for thick and thin sectioning of resin-embedded specimens. With good lighting, precise orientation of specimen and knife, plus the control of approach and feed from a separate unit, the UCT will cut sections as thin as 50nm for TEM or quickly cut 1-5 micron sections for light microscopy. Nearby in the lab is a knife breaker for making glass knives. We also have a cryo-chamber for sectioning at temperatures below -100C.

Instrument Access: Assisted use and training for individual use are available. To schedule assisted use or training, please fill out the Access Request Form and email it to Michelle Mikesh.

Fees: Trained users: $8/hour. Assisted use: $68/hour


Vibratome & Paraffin Microtome

The CBRS Microscopy and Imaging Facility has a Vibratome 1000 Plus Sectioning System and a Spencer 820 Paraffin Microtome available for sample sectioning.

Instrument Access: Training for individual use is available. To schedule training, please fill out the Access Request Form and email it to Michelle Mikesh.

Fees: Free to use after training.

Zeiss PALM Laser Microdissection

The Zeiss PALM is able to cut and extract regions of a tissue using a UV (355nm) cutting beam. Following dissection of tissue, the beam is used to catapult the region of interest into a tube where a number of downstream assays can then be performed.

Instrument Access: Training for individual use is available. To schedule training, please fill out the Access Request Form and email it to Anna Webb.

Fees: Trained users: $30/hour